Skip Navigation
ChemIDplus Lite•Browse•Advanced

Substance Name: Fluorescein [USP:BAN:JAN]
RN: 2321-07-5
UNII: TPY09G7XIR
InChIKey: GNBHRKFJIUUOQI-UHFFFAOYSA-N

Note

  • A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.

Molecular Formula

  • C20-H12-O5

Molecular Weight

  • 332.3098
 

Classification Codes

  • Coloring Agents
  • Contrast Media
  • Diagnostic Aid (Corneal Trauma Indicator)
  • Diagnostic Uses of Chemicals
  • Fluorescent Dyes
  • Indicators and Reagents
  • Luminescent Agents
  • Mutation Data
* denotes mobile formatted website

Links to Resources

NLM Resources (File Locators)

Regulatory Agencies (Superlist Locators)

Other Resources (Internet Locators)


Search for this InChIKey on the Web

Names and Synonyms

Name of Substance

  • 3',6'-Dihydroxyspiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one
  • CI 45350:1
  • D & C Yellow no. 7
  • Fluorescein
  • Fluorescein [USP:BAN:JAN]
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-

MeSH Heading

  • Fluorescein

Synonyms

  • 11712 Yellow
  • 3',6'-Dihydroxyfluoran
  • 3',6'-Dihydroxyspiro(isobenzofuran-1(3H),9'(9H)-xanthen)-3-one
  • 3,6-Dihydroxyspiro(xanthene-9,3'-phthalide)
  • 3,6-Fluorandiol
  • 3H-Xanthen-3-one, 9-(o-carboxyphenyl)-6-hydroxy-
  • 5-19-06-00456 (Beilstein Handbook Reference)
  • 9-(o-Carboxyphenyl)-6-hydroxy-3-isoxanthenone
  • 9-(o-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one
  • Benzoic acid, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-
  • Benzoic acid, o-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-
  • BRN 0094324
  • C.I. 45350:1
  • C.I. Solvent Yellow 94
  • CCRIS 7076
  • CI 45350:1
  • D and C Yellow No. 7
  • D+C Yellow No. 7
  • EC 219-031-8
  • EINECS 219-031-8
  • Fluoran, 3',6'-dihydroxy-
  • Fluorescein
  • Fluorescein acid
  • Fluorescein Red
  • Fluoresceine
  • Fluorescite
  • Hidacid fluorescein
  • HSDB 2128
  • Japan Yellow 201
  • Japan Yellow No. 201
  • NSC 667256
  • Resorcinolphthalein
  • Soap Yellow F
  • Solvent Yellow 94
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one,3'6'-dihydroxy-
  • UNII-TPY09G7XIR
  • Yellow fluorescein
  • Zlut kysela 73
  • Zlut kysela 73 [Czech]

Systematic Names

  • 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid
  • D&C Yellow No. 7
  • Fluorescein
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-

Superlist Name

  • Fluorescein

Registry Numbers

CAS Registry Number

  • 2321-07-5

FDA UNII

  • TPY09G7XIR

Other Registry Numbers

  • 126605-73-0
  • 213880-86-5
  • 518-46-7
  • 770677-61-7

Related Registry Numbers

  • 518-47-8 (di-hydrochloride salt)
  • 6417-85-2 (di-potassium salt)

System Generated Number

  • 0002321075

Structure Descriptors

InChI

InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChIKey

GNBHRKFJIUUOQI-UHFFFAOYSA-N

Smiles

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

Toxicity

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
guinea pig LDLo unreported 400mg/kg (400mg/kg)   "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1289, 1935.
man TDLo intravenous 7143nL/kg (7143mL/kg) SKIN AND APPENDAGES (SKIN): "DERMATITIS, OTHER: AFTER SYSTEMIC EXPOSURE"

GASTROINTESTINAL: NAUSEA OR VOMITING		 American Journal of Ophthalmology. Vol. 126, Pg. 837, 1998.
mouse LD50 intravenous 300mg/kg (300mg/kg)   United States Patent Document. Vol. #4775625,
mouse LDLo unreported 600mg/kg (600mg/kg)   "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1289, 1935.
rabbit LDLo intravenous 300mg/kg (300mg/kg)   International Journal of Leprosy and Other Mycobacterial Diseases. Vol. 2, Pg. 257, 1934.
rat LDLo intraperitoneal 600mg/kg (600mg/kg)   International Journal of Leprosy and Other Mycobacterial Diseases. Vol. 2, Pg. 257, 1934.

Physical Properties

Physical Property Value Units Temp (deg C) Source
Melting Point 315 dec deg C   EXP
log P (octanol-water) 3.350 (none)   EST
Atmospheric OH Rate Constant 2.00E-10 cm3/molecule-sec 25 EST

Physical property data is provided to ChemIDplus by SRC, Inc.