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Substance Name: Trypan Blue
RN: 72-57-1
UNII: I2ZWO3LS3M
InChIKey: GLNADSQYFUSGOU-GPTZEZBUSA-J

Note

  • A diazo-naphthalene sulfonate that is widely used as a stain.

Molecular Formula

  • C34-H28-N6-O14-S4.4Na

Molecular Weight

  • 960.8156
 

Classification Codes

Classification Codes

  • Coloring Agents
  • Drug / Therapeutic Agent
  • Mutation Data
  • Reproductive Effect
  • Tumor Data

Superlist Classification Codes

  • Overall Carcinogenic Evaluation: Group 2B
  • Reportable Quantity (RQ) = 10 lb

Names and Synonyms

Name of Substance

  • Trypan Blue

MeSH Heading

  • Trypan Blue

Synonyms

  • AI3-26698
  • Amanil Sky Blue R
  • Azidinblau 3B
  • Azidine Blue 3B
  • Azurro diretto 3B
  • Bencidal Blue 3B
  • Benzaminblau 3B
  • Benzamine Blue
  • Benzamine Blue 3B
  • Benzanil Blue 3BN
  • Benzanil Blue R
  • Benzo Blue
  • Benzo Blue 3B
  • Benzo Blue 3BS
  • Benzoblau 3B
  • Bleu diamine
  • Bleu diazole N 3B
  • Bleu directe 3B
  • Bleu trypane N
  • Bleue diretto 3B
  • Blue 3B
  • Blue EMB
  • Brasilamina Blue 3B
  • Brasilazina Blue 3B
  • C.I. 23850
  • C.I. Direct Blue 14
  • C.I. Direct Blue 14, tetrasodium salt
  • CCRIS 616
  • Centraline Blue 3B
  • Chloramiblau 3B
  • Chloramine Blue
  • Chloramine Blue 3B
  • Chlorazol Blue 3B
  • Chrome Leather Blue 3B
  • Congo Blue
  • Congoblau 3B
  • Cresotine Blue 3B
  • Diaminblau 3B
  • Diamine Blue 3B
  • Diamineblue
  • Diaminine Blue
  • Dianil Blue
  • Dianil Blue H3G
  • Dianilblau
  • Dianilblau H3G
  • Diaphtamine Blue TH
  • Diazine Blue 3B
  • Diazol Blue 3B
  • Diphenyl Blue 3B
  • Direct Blue 14
  • Direct Blue 3B
  • Direct Blue 3BX
  • Direct Blue D3B
  • Direct Blue H3G
  • Direct Blue M3B
  • Directakol Blue 3BL
  • Directblau 3B
  • EINECS 200-786-7
  • Hispamin Blue 3BX
  • HSDB 2945
  • Membraneblue
  • Modr Prima 14
  • Modr Prima 14 [Czech]
  • Modr Trypanova
  • Modr Trypanova [Czech]
  • Naphthaminblau 3BX
  • Naphthamine Blue 3BX
  • Naphthylamine Blue
  • NCI C61289
  • Niagara Blue
  • Niagara Blue 3B
  • NSC 11247
  • Orion Blue 3B
  • Paramine Blue 3B
  • Parkibleu
  • Parkipan
  • Pontamine Blue 3BX
  • Pyrazol Blue 3B
  • Pyrotropblau
  • RCRA waste number U236
  • Renolblau 3B
  • Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate
  • Sodium ditolyldisazobis-8-amino-1-naphthol-3,6-disulfonate
  • Sodium ditolyldisazobis-8-amino-1-naphthol-3,6-disulphonate
  • Trianol Direct Blue 3B
  • Triazolblau 3BX
  • Tripan Blue
  • Trypan (Congo) Blue
  • Trypan Blue
  • Trypan Blue BPC
  • Trypanblau
  • Trypanblau [German]
  • Trypane Blue
  • UNII-I2ZWO3LS3M
  • Vision Blue
  • VisionBlue

Systematic Names

  • 2,7-Naphthalenedisulfonic acid, 3,3'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(2,1-diazenediyl))bis(5-amino-4-hydroxy-, sodium salt (1:4)
  • 2,7-Naphthalenedisulfonic acid, 3,3'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(azo))bis(5-amino-4-hydroxy-, tetrasodium salt
  • C.I. Direct Blue 14
  • C.I. Direct Blue 14, tetrasodium salt
  • Tetrasodium 3,3'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(azo))bis(5-amino-4-hydroxynaphthalene-2,7-disulphonate)

Superlist Names

  • 2,7-Naphthalenedisulfonic acid, 3,3'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(azo))bis(5-amino-4-hydroxy-, tetrasodium salt
  • RCRA waste no. U236
  • Trypan Blue
  • Trypan blue (commercial grade)

Registry Numbers

CAS Registry Number

  • 72-57-1

FDA UNII

  • I2ZWO3LS3M

Other Registry Number

  • 179472-55-0

System Generated Number

  • 0000072571

Molecular Formulas

Molecular Formula

  • C34-H28-N6-O14-S4.4Na

Molecular Formula Fragments

  • C34-H28-N6-O14-S4
  • COMPONENT
  • Na

Structure Descriptors

InChI

InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;

InChIKey

GLNADSQYFUSGOU-GPTZEZBUSA-J

Smiles

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2c(O)c3c(N)cc(cc3cc2S(=O)(=O)[O-])S(=O)(=O)[O-])c4ccc(\N=N\c5c(O)c6c(N)cc(cc6cc5S(=O)(=O)[O-])S(=O)(=O)[O-])c(C)c4

Toxicity

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
guinea pig LDLo intraperitoneal 250mg/kg (250mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
guinea pig LDLo subcutaneous 300mg/kg (300mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
mouse LD50 intravenous 328mg/kg (328mg/kg)   Toxicology and Applied Pharmacology. Vol. 23, Pg. 537, 1972.
mouse LD50 subcutaneous 267mg/kg (267mg/kg)   Naunyn-Schmiedebergs Archiv fuer Pharmakologie. Vol. 267, Pg. 31, 1970.
mouse LDLo intraperitoneal 350mg/kg (350mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
rabbit LDLo intraperitoneal 400mg/kg (400mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
rabbit LDLo intravenous 100mg/kg (100mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
rat LD50 oral 6200mg/kg (6200mg/kg)   Office of Toxic Substances Report. Vol. OTS,
rat LDLo intraperitoneal 300mg/kg (300mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
rat LDLo intravenous 300mg/kg (300mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.
rat LDLo subcutaneous 300mg/kg (300mg/kg) LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA Proceedings of the Society for Experimental Biology and Medicine. Vol. 31, Pg. 825, 1934.

Physical Properties

Physical Property Value Units Temp (deg C) Source
Melting Point >300 deg C   EXP
log P (octanol-water) -0.120 (none)   EST
Atmospheric OH Rate Constant 2.27E-11 cm3/molecule-sec 25 EST

Physical property data is provided to ChemIDplus by SRC, Inc.